Anti-RAN antibody
种属反应性Human,Mouse,Monkey,Rat
验证应用WB,IHC-P,ICC,FC
抗体类型小鼠单抗
免疫原Recombinant protein
偶联Non-conjugated
Anti-RAN antibody性能
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG1
纯化方式Protein A purified.
亚细胞定位Nucleus. Cytoplasm. Melanosome.
其它名称
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Anti-RAN antibody应用
WB: 1:500-1:2,000
ICC: 1:200-1:500
IHC-P: 1:200-1:500
FC: 1:100-1:200
Fig1: Western blot analysis of RAN on human RAN recombinant protein using anti-RAN antibody at 1/1,000 dilution.
Fig2: Western blot analysis of RAN on HEK293 (1) and RAN-hIgGFc transfected HEK293 (2) cell lysate using anti-RAN antibody at 1/1,000 dilution.
Fig3: Western blot analysis of RAN on different cell lysate using anti-RAN antibody at 1/1,000 dilution.
Positive control: Line1: Hela Line2: NIH/3T3 Line3: A431 Line4: C6 Line5: Jurkat Line6: Hela Line7: COS7 Line8: Jurkat
Fig4: ICC staining RAN (green) and Actin filaments (red) in GC-7901 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: ICC staining RAN (green) and Actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig6: ICC staining RANRAN (green) and Actin filaments (red) in HepG2 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig7: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-RAN antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-RAN antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of Hela cells with RAN antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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