Fig3: Flow cytometric analysis of TNFRSF19 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody ( (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).