Anti-Smad2 antibody
种属反应性Human,Mouse,Rat
验证应用WB,IHC-P,ICC
抗体类型小鼠单抗
免疫原Recombinant protein
偶联Non-conjugated
Anti-Smad2 antibody性能
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG1
纯化方式Protein G purified.
亚细胞定位Cytoplasm. Nucleus.
其它名称
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Anti-Smad2 antibody应用
WB: 1:500
ICC: 1:50-1:200
IHC-P: 1:50-1:200
Fig1: Western blot analysis of Smad2 on Hela cell lysate using anti-Smad2 antibody at 1/500 dilution.
Fig2: ICC staining Smad2 (green) in HUVEC cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Smad2 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Smad2 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Smad2 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Smad2 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Smad2 antibody. Counter stained with hematoxylin.
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