Anti-CD134/OX40 antibody
种属反应性Human,Mouse,Rat
验证应用WB,IHC-P,ICC,FC
抗体类型小鼠单抗
免疫原Recombinant protein
偶联Non-conjugated
Anti-CD134/OX40 antibody性能
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG1
纯化方式Protein A purified.
亚细胞定位Membrane.
其它名称
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ACT 35 antibody
ACT35 antibody
ACT35 antigen antibody
Anti-CD134/OX40 antibody应用
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
WB: 1:500
Fig1: ICC staining CD134 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2: ICC staining CD134 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining CD134 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-CD134 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CD134 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD134 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD134 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD134 antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of HL-60 cells with CD134 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary
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