Anti-Grp75 antibody
种属反应性Human,Mouse,Rat
验证应用WB,IHC-P,ICC
抗体类型小鼠单抗
免疫原Peptide
偶联Non-conjugated
Anti-Grp75 antibody性能
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG1
纯化方式Protein A purified.
亚细胞定位Mitochondrion. Nucleus.
其它名称
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Anti-Grp75 antibody应用
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
Fig1: Western blot analysis of Grp75 on different cell lysates using anti-Grp75 antibody at 1/1,000 dilution.
Positive control:
Lane 1: NIH-3T3
Lane 2: HepG2
Lane 3: K562
Lane 4: L929
Fig2: ICC staining Grp75 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Grp75 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Grp75 (green) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Grp75 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Grp75 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse oviduct tissue using anti-Grp75 antibody. Counter stained with hematoxylin.
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