Fig3: Western blot analysis of TNFSF11 against COS7 (1), Hela (2), U937 (3), HL-60 (4), Raji (5), Ramos (6), Jurkat (7), and SW480 (8) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.