Anti-ADORA2A antibody
种属反应性Human
验证应用WB,ICC,FC
抗体类型小鼠单抗
免疫原Purified recombinant fragment of human ADORA2A (AA: 274-412) expressed in E. Coli.
偶联Non-conjugated
形态Liquid
浓度1 mg/mL
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS with 0.05% sodium azide.
亚型IgG2a
纯化方式Protein G purified.
亚细胞定位Cell membrane.
其它名称
A2AAR antibody
A2aR antibody
AA2AR_HUMAN antibody
WB: 1:500-1:2,000
ICC: 1:50-1:200
FC: 1:100-1:200
Fig1: Western blot analysis of ADORA2A against human ADORA2A (AA: 274-412) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Western blot analysis of ADORA2A against HEK293 (1) and ADORA2A (AA: 274-412)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Fig3: Immunocytochemistry staining of ADORA2A in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue), Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Fig4: Flow cytometric analysis of ADORA2A was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
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企业名称
上海泽叶生物科技有限公司
企业信息已认证
企业类型
信用代码
91310116MAJ8N934E
成立日期
2016-08-09
注册资本
100
经营范围
生物科技
上海泽叶生物科技有限公司
公司地址
上海市松江区国家经济开发区北松公路5629号聚科生物松江园区
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