Anti-AKT1 antibody
种属反应性Human,Mouse
验证应用WB,ICC,IHC-P,FC
抗体类型小鼠单抗
免疫原peptide
偶联Non-conjugated
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG2b
纯化方式Protein A purified.
亚细胞定位Nucleus, cytoplasm, cell membrane.
其它名称
AKT 1 antibody
AKT antibody
AKT1 antibody
WB: 1:2,000
IHC-P: 1:100-1:200
ICC: 1:100-1:200
FC: 100-1:200
Fig1: Western blot analysis of Akt1 on different cell lysates using anti-Akt1 antibody at 1/2000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: MCF-7
Lane 3: Hela
Fig2: ICC staining Akt1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Akt1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Akt1 in MCF-7 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Akt1 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of Hela cells with Akt1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
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企业名称
上海泽叶生物科技有限公司
企业信息已认证
企业类型
信用代码
91310116MAJ8N934E
成立日期
2016-08-09
注册资本
100
经营范围
生物科技
上海泽叶生物科技有限公司
公司地址
上海市松江区国家经济开发区北松公路5629号聚科生物松江园区
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