Anti-GRIK4 antibody

Anti-GRIK4 antibody

• 种属反应性Human

• 验证应用WB,IHC,FC

• 抗体类型兔多抗

• 免疫原Synthetic peptide within human GRIK4 aa 30-80.

• Anti-GRIK4 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified.

• 亚细胞定位Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse.

  
  

• 其它名称

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• EAA 1 antibody

• EAA1 antibody

• Excitatory amino acid receptor 1 antibody

• Anti-GRIK4 antibody应用

  WB: 1:500
  IHC: 1:50-1:200
  FC: 1:50-1:100

• 

  Fig1: Western blot analysis of GRIK4 on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue using anti-GRIK4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig3: Flow cytometric analysis of GRIK4 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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