Anti-ENAH antibody
种属反应性Human,Mouse,Rat
验证应用WB
抗体类型兔多抗
免疫原Synthetic peptide within C-terminal human ENAH.
偶联Non-conjugated
Anti-ENAH antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified.
亚细胞定位Cytoskeleton, cytoplasm, lamellipodium, filopodium, synapse, focal adhesion.
其它名称
more
ENA antibody
Enah antibody
ENAH_HUMAN antibody
Anti-ENAH antibody应用
WB: 1:500-1:2,000
Fig1: Western blot analysis of ENAH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: Hela cell lysate
Lane 3: 293T cell lysate
Fig2: Western blot analysis of ENAH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: NIH/3T3 cell lysate
Lane 2: Rat brain tissue lysate
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