Anti-Perilipin-5 antibody
种属反应性Human,Mouse
验证应用WB,IHC-P,FC
抗体类型兔多抗
免疫原Recombinant protein corresponding to N-terminal human Perilipin-5.
偶联Non-conjugated
Anti-Perilipin-5 antibody性能
形态Liquid
浓度1 mg/ml.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Protein affinity purified.
亚细胞定位Cytoplasm, Lipid droplet, Mitochondrion.
其它名称
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Anti-Perilipin-5 antibody应用
WB: 1:500-1:1,000
IHC-P: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of Perilipin-5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human liver tissue lysate
Lane 2: Human marrow tissue lysate
Fig2: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Perilipin-5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig3: Flow cytometric analysis of Perilipin-5 was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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