Anti-Beta-adaptin-like protein A antibody
种属反应性Arabidopsis thaliana
验证应用IHC-P,FC
抗体类型兔多抗
免疫原Synthetic peptide within A. thaliana Beta-adaptin-like protein A aa 770-841.
偶联Non-conjugated
Anti-Beta-adaptin-like protein A antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified
亚细胞定位Golgi apparatus, trans-Golgi network, clathrin-coated vesicle membrane.
其它名称
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Anti-Beta-adaptin-like protein A antibody应用
IHC-P:1:50-1:200
FC:1:50-1:100
Fig1: Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-Beta-adaptin-like protein A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig2: Immunofluorescence staining of paraffin- embedded A. thaliana using anti-Rubisco activase rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
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