Anti-Cathepsin D antibody
种属反应性Human,Mouse
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原Peptide
偶联Non-conjugated
Anti-Cathepsin D antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified
亚细胞定位Lysosome. Melanosome. Secreted, extracellular space.
其它名称
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CatD antibody
CATD_HUMAN antibody
Cathepsin D antibody
Anti-Cathepsin D antibody应用
WB: 1:500
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:200
Fig1: Western blot analysis of Cathepsin D on MCF-7 cell lysate using anti-Cathepsin D antibody at 1/1,000 dilution.
Fig2: ICC staining Cathepsin D in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Cathepsin D in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Cathepsin D in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of HepG2 cells with Cathepsin D antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Fig8: Flow cytometric analysis of MCF-7 cells with Cathepsin D antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
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