Anti-SHE antibody

Anti-SHE antibody

• 种属反应性Zebrafish

• 验证应用WB,IF,IHC-P

• 抗体类型兔多抗

• 免疫原Recombinant protein within Zebrafish SHE aa 80-280.

• 偶联Non-conjugated

• Anti-SHE antibody性能

• 形态Liquid

• 浓度1 mg/ml.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Protein affinity purified.

• 亚细胞定位Cytoplasm, Membrane. (Predicted)

• 其它名称

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• RP11-350G8.8 antibody

• SH2 domain-containing adapter protein E antibody

• She antibody

• Anti-SHE antibody应用

  WB: 1:500-1:1000
  IF: 1:50-1:200
  IHC-P: 1:50-1:200

• 

  Fig1: Immunofluorescence staining of paraffin- embedded Zebrafish using anti-SHE rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody  at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

  

  Fig2: Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-SHE antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody  at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig3: Western blot analysis of SHE on Zebrafish tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

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