Anti-Mono-mehtyl-Histone H3(Lys4) antibody
种属反应性Human,Mouse,Rat
验证应用WB,ICC,IHC,FC
抗体类型兔多抗
免疫原peptide
偶联Non-conjugated
Anti-Mono-mehtyl-Histone H3(Lys4) antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Immunogen affinity purified
亚细胞定位Nucleus
其它名称
more
HIST1 cluster, H3J antibody
Histone gene cluster 1, H3 histone family, member E antibody
Histone gene cluster 1, H3G antibody
Anti-Mono-mehtyl-Histone H3(Lys4) antibody应用
WB: 1:500-1:2000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of Mono-mehtyl-Histone H3(Lys4) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
A: NCCIT
B: purified 293T Histone
C: F9
D: F9 with Histone H3 peptide-unmodifed
E: F9 with Histone H3 peptide-mono methyl K4 lysates using anti-Histone H3(mono methyl K4) polyclonal antibody.
Fig2: ICC staining of Mono-mehtyl-Histone H3(Lys4) in NCCIT cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Mono-mehtyl-Histone H3(Lys4) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Mono-mehtyl-Histone H3(Lys4) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig5: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Mono-mehtyl-Histone H3(Lys4) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig6: Flow cytometric analysis of Mono-mehtyl-Histone H3(Lys4) was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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