Anti-CD3 epsilon antibody

Anti-CD3 epsilon antibody

• 种属反应性Human,Mouse

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to N-terminal residues of CD3 epsilon.

• 偶联Non-conjugated

• Anti-CD3 epsilon antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Membrane.

• 其它名称CD3 epsilon antibody
  CD3e antibody
  CD3e antigen epsilon polypeptide (TiT3 complex) antibody
  CD3E antigen epsilon polypeptide antibody
  CD3E antigen, epsilon subunit antibody
  CD3e molecule epsilon antibody
  CD3e molecule, epsilon (CD3 TCR complex) antibody
  CD3e molecule, epsilon (CD3-TCR complex) antibody
  CD3E_HUMAN antibody
  IMD18 antibody
  T cell antigen receptor complex epsilon subunit of T3 antibody
  T cell surface antigen T3/Leu 4 epsilon chain antibody
  T cell surface glycoprotein CD3 epsilon chain antibody
  T-cell surface antigen T3/Leu-4 epsilon chain antibody
  T-cell surface glycoprotein CD3 epsilon chain antibody
  T3E antibody
  TCRE antibody

  more

• Anti-CD3 epsilon antibody应用

  WB: 1:1,000
  ICC: 1:200
  IHC-P: 1:200
  FC: 1:100-1:200

• 

  Fig1: Western blot analysis of CD3E on different cell lysates using anti-CD3E antibody at 1/1000 dilution.

  

  Fig2: ICC staining CD3E in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: ICC staining CD3E in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD3E antibody. Counter stained with hematoxylin.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD3E antibody. Counter stained with hematoxylin.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD3E antibody. Counter stained with hematoxylin.

  

  Fig7: Flow cytometric analysis of Jurkat cells with CD3E antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

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