Fig1: Western Blot analysis of 22RV-1, BT474, SHSY5Y cells using FGF-12 Polyclonal Antibody.. Secondary antibody(catalog#:HA1001) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).