Anti-FGF-18 antibody

Anti-FGF-18 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原The antiserum was produced against synthesized peptide derived from the C-terminal region of human FGF18. AA range:158-207.

• 偶联Non-conjugated

• Anti-FGF-18 antibody性能

• 形态Liquid

• 浓度1 mg/ml.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 亚型IgG

• 纯化方式Peptide affinity purified.

• 亚细胞定位Secreted.

• 其它名称FGF 18 antibody
  FGF-18 antibody
  Fgf18 antibody
  FGF18_HUMAN antibody
  FGFI antibody
  Fibroblast growth factor 18 antibody
  zFGF5 antibody

  more

• Anti-FGF-18 antibody应用

  WB: 1:500-2,000
  IHC-P: 1:50-1:200

• 

  Fig1: Western blot analysis of FGF18 on human small intestine tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER60001, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: SW480 cell lysates
  Lane 2: SKOV3 cell lysates
  Lane 3: BT474 cell lysates
  Lane 4: Mouse heart tissue lysates

  

  Fig2: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-FGF18 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER60001, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX

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