Anti-Topoisomerase II alpha antibody
种属反应性Human,Mouse,Rat
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原peptide
偶联Non-conjugated
Anti-Topoisomerase II alpha antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified
亚细胞定位Cytoplasm, nucleus.
其它名称alpha isozyme antibody
ATP hydrolyzing DNA topoisomerase II alfa antibody
DNA gyrase antibody
DNA topoisomerase (ATP hydrolyzing) antibody
DNA topoisomerase 2 alpha antibody
DNA topoisomerase 2-alpha antibody
DNA topoisomerase II 170 kD antibody
DNA topoisomerase II alpha isozyme antibody
DNA topoisomerase II antibody
DNA Topoisomerase2 antibody
TOP 2A antibody
TOP2 antibody
TOP2A antibody
TOP2A_HUMAN antibody
Topoisomerase DNA II alpha 170kDa antibody
TP2A antibody
more
Anti-Topoisomerase II alpha antibody应用
WB: 1:500
ICC: 1:200
IHC-P: 1:200
FC: 1:100-1:200
Fig1: Western blot analysis of TOP2A on different cell lysates using anti-TOP2A antibody at 1/500 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: NIH/3T3
Lane 3: A431
Lane 4: L929
Lane 5: A549
Lane 6: Human liver
Lane 7: HUVEC
Fig2: ICC staining TOP2A in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining TOP2A in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining TOP2A in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: ICC staining TOP2A in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of Hela cells with TOP2A antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
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