Anti-PDI antibody

Anti-PDI antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,IHC-P

• 抗体类型兔多抗

• 免疫原peptide

• 偶联Non-conjugated

• Anti-PDI antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Endoplasmic reticulum lumen

• 其它名称Pancreas specific protein disulfide isomerase antibody
  Pancreas-specific protein disulfide isomerase antibody
  Pancreatic protein disulfide isomerase antibody
  PDA2 antibody
  PDI antibody
  PDIA2 antibody
  PDIA2_HUMAN antibody
  PDIp antibody
  PDIR antibody
  Protein disulfide isomerase A2 antibody
  Protein disulfide isomerase antibody
  Protein disulfide isomerase associated 2 antibody
  Protein disulfide isomerase family A member 2 antibody
  Protein disulfide isomerase pancreatic antibody
  Protein disulfide-isomerase A2 antibody
  Rho GDP dissociation inhibitor gamma antibody

  more

• Anti-PDI antibody应用

  WB: 1:500-1:1,000
  ICC: 1:200
  IHC-P: 1:200

• 

  Fig1: Western blot analysis of PDI on different cell lysates using anti- PDI antibody at 1/1000 dilution.
  Positive control:
  Lane 1: Hela     Lane 2: MCF-7
  Lane 3: PANC     Lane 4: HepG2
  Lane 5: HUVEC     Lane 6: NIH/3T3
  Lane 7: L929     Lane 8: F9
  Lane 9: Jurkat     Lane 10: A431
  Lane 11: A549     Lane 12: Mouse liver
  Lane 13: Mouse kidney   Lane 14: Mouse brain
  Lane 15: Human kidney   Lane 16: Human brain
  Lane 17: Human liver

  

  Fig2: ICC staining PDI in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: ICC staining PDI in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig4: ICC staining PDI in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-PDI antibody. Counter stained with hematoxylin.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PDI antibody. Counter stained with hematoxylin.

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