Anti-CDk1 antibody

Anti-CDk1 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原peptide

• 偶联Non-conjugated

• Anti-CDk1 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Cytoplasm, nucleus

• 其它名称Cdc 2 antibody
  Cdc2 antibody
  CDC28A antibody
  CDK 1 antibody
  CDK1 antibody
  CDK1_HUMAN antibody
  CDKN1 antibody
  CELL CYCLE CONTROLLER CDC2 antibody
  Cell division control protein 2 antibody
  Cell division control protein 2 homolog antibody
  Cell division cycle 2 G1 to S and G2 to M antibody
  Cell division protein kinase 1 antibody
  Cell Divsion Cycle 2 Protein antibody
  Cyclin Dependent Kinase 1 antibody
  Cyclin-dependent kinase 1 antibody
  DKFZp686L20222 antibody
  MGC111195 antibody
  p34 Cdk1 antibody
  p34 protein kinase antibody
  P34CDC2 antibody

  more

• Anti-CDk1 antibody应用

  WB: 1:500
  ICC: 1:200
  IHC-P: 1:200
  FC: 1:100-1:200

• 

  Fig1: Western blot analysis of CDk1 on different cell lysates using anti-CDk1 antibody at 1/500 dilution.
  Positive control:
  Lane 1: MCF-7
  Lane 2: Jurkat
  Lane 3: PC12
  Lane 4: HepG2
  Lane 5: Hela
  Lane 6: NIH/3T3
  Lane 7: Mouse liver
  Lane 8: SKBR3

  

  Fig2: ICC staining CDk1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: ICC staining CDk1 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig4: ICC staining CDk1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CDk1 antibody. Counter stained with hematoxylin.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CDk1 antibody. Counter stained with hematoxylin

  

  Fig7: Immunohistochemical analysis of paraffin-e.mbedded human breast cancer tissue using anti-CDk1 antibody. Counter stained with hematoxylin

  

  Fig8: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CDk1 antibody. Counter stained with hematoxylin.

  

  Fig9: Flow cytometric analysis of Hela cells with CDk1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

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