Anti-Stat-1 alpha/beta antibody
种属反应性Human,Mouse
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原peptide
偶联Non-conjugated
Anti-Stat-1 alpha/beta antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified
亚细胞定位Cytoplasm, nucleus.
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Anti-Stat-1 alpha/beta antibody应用
WB: 1:1,000
ICC: 1:200
IHC-P: 1:200,
FC: 1:100-1:200
Fig1: Western blot analysis of Stat-1α/β on different cell lysates using anti-Stat-1α/β antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: NIH/3T3
Lane 3: MCF-7
Fig2: ICC staining Stat-1α/β in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Stat-1α/β in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Stat-1α/β in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Stat-1α/β antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse skeletal human kidney tissue using anti-Stat-1α/β antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Stat-1α/β antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of Hela cells with Stat-1α/β antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
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