Anti-Ubiquitin antibody
种属反应性Human,Mouse,Rat
验证应用WB,ICC,IHC-P
抗体类型兔多抗
免疫原peptide
偶联Non-conjugated
Anti-Ubiquitin antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified
亚细胞定位Cytoplasm, nucleus
其它名称Epididymis secretory protein Li 50 antibody
FLJ25987 antibody
HEL S 50 antibody
MGC8385 antibody
Polyubiquitin B antibody
RPS 27A antibody
RPS27A antibody
UBA 52 antibody
UBA 80 antibody
UBA52 antibody
UBA80 antibody
UBB antibody
UBB_HUMAN antibody
UBC antibody
UBCEP 1 antibody
UBCEP 2 antibody
UBCEP1 antibody
UBCEP2 antibody
Ubiquitin antibody
Ubiquitin B antibody
more
Anti-Ubiquitin antibody应用
WB: 1:500-1:1,000
ICC: 1:200
IHC-P: 1:200
Fig1: Western blot analysis of Ubiquitin on different cell lysates using anti-Ubiquitin antibody at 1/1000 dilution.
Positive control:
Lane 1: 293T
Lane 2: HepG2
Lane 3: Hela
Lane 4: MCF-2
Fig2: ICC staining Ubiquitin in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Ubiquitin in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Ubiquitin in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Ubiquitin antibody. Counter stained with hematoxylin.
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