种属反应性Human,Mouse,Rat,Dog,Pig,Horse,Sheep
验证应用WB,IHC-P
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human TRIM23 201-300/574
偶联Non-conjugated
Anti-TRIM23 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Endomembrane system. Golgi apparatus membrane. Lysosome membrane. Note: Membrane associated with the Golgi complex and lysosomal structures.
其它名称ADP ribosylation factor domain protein 1
ARF domain protein 1
ARFD1
GTP binding protein ARD 1
TRI23_HUMAN
N-acetyltransferase ARD1 human homolog of
N-acetyltransferase homolog of S. cerevisiae ARD1
TRIM23
Tripartite motif protein TRIM23.
more
Anti-TRIM23 antibody应用
WB:1:500-2000
IHC-P:1:400-800
Fig1: Sample: 293T (Human)Cell Lysate at 40 ug
Primary: Anti-TRIM23at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD
Fig2: Sample: Hela (Human) Cell Lysate at 40 ug
Primary: Anti-TRIM23at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD
Fig3: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TRIM23) Polyclonal Antibody, Unconjugated ( at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig4: Sample: A549 Cell (Human) Lysate at 40 ug
Primary: Anti-TRIM23 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD
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