Anti-TREM1 antibody

Anti-TREM1 antibody

• 种属反应性Human,Rat

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human TREM1 151-234/234

• 偶联Non-conjugated

• Anti-TREM1 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式Affinity purified by Protein A

• 亚细胞定位Isoform 1: Cell membrane; Single-pass type I membrane protein (Potential). Isoform 2: Secreted (Potential).

• 其它名称TREM 1
  TREM-1
  Triggering receptor expressed on monocytes 1
  Triggering receptor expressed on myeloid cells 1
  Triggering receptor TREM 1
  Triggering receptor TREM1
  Triggering-receptor TREM 1
  Triggering-receptor TREM-1
  Triggering-receptor TREM1
  TREM1_HUMAN
  CD354.

  more

• Anti-TREM1 antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg/Test

• 

  Fig1: Primary Antibody:Rabbit anti-TREM1 antibody Supplier catalog number:  Supplier: Bioss Isotype Control Antibody: Rabbit IgG Secondary Antibody: F(ab’)2 fragment goat anti-rabbit IgG-PE Supplier: Jackson Lab. Positive control:U937(4% paraformaldehyde for 10 min at room temperature)

  

  Fig2: Primary Antibody:Rabbit anti-TREM1 antibody Supplier catalog number:Supplier: Bioss Isotype Control Antibody: Rabbit IgG Secondary Antibody: F(ab’)2 fragment goat anti-rabbit IgG-PE Supplier: Jackson Lab. Positive control:U937(4% paraformaldehyde for 10 min at room temperature)

  

  Fig3: Sample:hcclm3(Human) Cell Lysate at 30 ug
  K562(Human) Cell Lysate at 30 ug
  Primary: Anti-CD200R (bs-7351R) at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 24 kD
  Observed band size: 21 kD
  

  

  Fig4: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TREM1) Polyclonal Antibody, Unconjugated ( at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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