Anti-Sema3A antibody

Anti-Sema3A antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from mouse Sema3A 22-120/772

• 偶联Non-conjugated

• Anti-Sema3A antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Secreted.

• 其它名称Semaphorin 3A
  Semaphorin-3A
  Semaphorin-3A precursor
  Semaphorin3A
  SemD
  Sema 3A
  SEM3A_MOUSE
  Coll 1
  Coll1
  Hsema I
  Hsema III
  sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3A
  Sema III
  SEMA1
  SEMA1-PEN
  SEMA3A
  SEMAD
  SEMAIII
  SEMAL
  Semaphorin III
  Semaphorin like growth cone guidance homolog of chick collapsin
  Semaphorin III
  Semaphorin-D
  Sema D.

  more

• Anti-Sema3A antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Sample:
  Lung (Mouse) Lysate at 40 ug
  Primary: Anti-Sema3Aat 1/500 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 86 kD
  Observed band size: 86 kD
  

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Sema3A) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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