种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Zebrafish
验证应用WB,IHC-P,ICC/IF
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human RNF128 151-260/428
偶联Non-conjugated
Anti-RNF128 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Endomembrane system; Single-pass membrane protein. Cytoplasm, perinuclear region. Note=Localized in an asymmetric perinuclear punctuate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the e
其它名称E3 ubiquitin protein ligase RNF128
E3 ubiquitin-protein ligase RNF128
FLJ23516
Gene related to anergy in lymphocytes protein
GRAIL
ring finger protein 128
RN128_HUMAN
RNF128.
more
Anti-RNF128 antibody应用
WB:1:500-2000
IHC-P:1:400-800
IF:1:50-200
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig4: Sample:
Cerebrum (Mouse) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
293T(Human) Cell Lysate at 30 ug
Ovary (Mouse) Lysate at 40 ug
Primary: Anti- RNF128 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 62 kD
Fig5: Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti- RNF128 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 61kD
Anti-RNF128 antibody信息由上海泽叶生物科技有限公司为您提供,如您想了解更多关于Anti-RNF128 antibody报价、型号、参数等信息,欢迎来电或留言咨询。除供应Anti-RNF128 antibody外,上海泽叶生物科技有限公司还可为您提供Anti-14-3-3 beta/alpha antibody、Anti-Caveolin-1 antibody、Anti-Junctophilin-2 antibody等产品,公司有专业的客户服务团队,是您值得信赖的合作伙伴。