Anti-REC8 antibody
种属反应性Human,Mouse,Rat,Dog,Pig,Horse,Rabbit,Guinea Pig
验证应用WB,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human REC8 22-120/547
偶联Non-conjugated
Anti-REC8 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Nucleus. Chromosome.
其它名称Cohesin Rec8p
Meiotic recombination protein REC8 homolog
Meiotic recombination protein REC8-like 1
rec8
REC8 homolog (yeast)
REC8_HUMAN
REC8L
Rec8L1
Rec8p.
more
Anti-REC8 antibody应用
WB:1:500-2000
FC:1ug/Test
Fig1: Blank control (Black line):Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-REC8 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Fig2: Sample: HT29 Cell (Human) Lysate at 40 ug
Primary: Anti-REC8 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 65 kD
Fig3: Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-REC8 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
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