Anti-RBM20 antibody
种属反应性Human,Mouse,Rat,Dog,Cow,Horse,Sheep
验证应用WB,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human RBM20 361-460/1227
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Nucleus (By similarity).
其它名称
WB:1:500-2000
FC:3ug/test
Fig1: Sample: heart (mouse) Lysate at 40 ug
Primary: Anti- RBM20 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 75kD
Observed band size: 75 kD
Fig2: Sample:
K562(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
Primary: Anti- RBM20 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 134 kD
Observed band size: 130 kD
Fig3: Blank control:A549.
Primary Antibody (green line): Rabbit Anti-RBM20 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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企业名称
上海泽叶生物科技有限公司
企业信息已认证
企业类型
信用代码
91310116MAJ8N934E
成立日期
2016-08-09
注册资本
100
经营范围
生物科技
上海泽叶生物科技有限公司
公司地址
上海市松江区国家经济开发区北松公路5629号聚科生物松江园区
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