Anti-Pro-neuregulin-1, membrane-bound isoform antibody

Anti-Pro-neuregulin-1, membrane-bound isoform antibody

• 种属反应性Human,Mouse,Rat,Chicken,Dog,Rabbit

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from mouse HRG Beta1 221-320/636

• 偶联Non-conjugated

• Anti-Pro-neuregulin-1, membrane-bound isoform antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Pro-neuregulin-1, membrane-bound isoform: Cell membrane; Single-pass type I membrane protein. Note=Does not seem to be active. Neuregulin-1: Secreted. Isoform 8: Nucleus. Note=May be nuclear. Isoform 9: Secreted. Note=Has a signal peptide. Isoform

• 其它名称Acetylcholine receptor-inducing activity
  ARIA
  Breast cancer cell differentiation factor p45
  CHICK, homolog of
  GGF
  GGF2
  Glial growth factor
  Heregulin
  heregulin, alpha (45kD, ERBB2 p185-activator)
  heregulin, alpha
  HGL
  HRG
  HRG1
  HRGA
  MST131
  NDF
  Neu differentiation factor
  neuregulin 1 type IV beta 1a
  neuregulin 1 type IV beta 3
  NRG 1
  nrg1
  NRG1 beta
  NRG1_HUMAN
  Pro-neuregulin-1, membrane-bound isoform
  Pro-NRG1
  Sensory and motor neuron-derived factor
  SMDF
  NRG1_MOUSE.

  more

• Anti-Pro-neuregulin-1, membrane-bound isoform antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:3ug/test

• 

  Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-HRG beta1 Polyclonal Antibody, Unconjugated(1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig2: Sample: Raji Cell Lysate at 30ug;
  Primary: Anti-HRG beta1 ( at 1:300 dilution;
  Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
  Predicted band size :71 kD
  Observed band size :71 kD
  

  

  Fig3: ue/cell: Mouse ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti- HRG Beta1 Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Pro-neuregulin-1, membrane-bound isoform) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

  

  Fig5: Blank control:A549.
  Primary Antibody (green line): Rabbit Anti-Pro-neuregulin-1, membrane-bound isoform antibody 
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody : Goat anti-rabbit IgG-PE
  Dilution: 3μg /test.
  Protocol
  The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

  

  Fig6: Blank control:A549.
  Primary Antibody (green line): Rabbit Anti-Pro-neuregulin-1, membrane-bound isoform antibody 
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody : Goat anti-rabbit IgG-PE
  Dilution: 3μg /test.
  Protocol
  The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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