Anti-Podoplanin antibody

Anti-Podoplanin antibody

• 种属反应性Mouse,Rat

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from mouse Podoplanin 91-166/166

• 偶联Non-conjugated

• Anti-Podoplanin antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Membrane; Plasma membrane.

• 其它名称Podoplanin /gp36
  Podoplanin Protein
  AGGRUS
  GLYCOPROTEIN 36 KD
  Podoplanin
  Glycoprotein 36
  gp 36
  GP 38
  GP 40
  gp36
  GP38
  GP40
  HT1A 1
  HT1A1
  hT1alpha1
  hT1alpha2
  Lung type I cell membrane associated glycoprotein
  Lung type I cell membrane associated glycoprotein isoform a
  Lung type I cell membrane associated glycoprotein T1A 2
  OTS 8
  OTS8
  OTTHUMP00000009640
  OTTHUMP00000044504
  PA2.26
  PA2.26 antigen
  PDPN
  Podoplanin
  T1 alpha
  T1 ALPHA GENE
  T1A
  TIA 2
  TIA2.

  more

• Anti-Podoplanin antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Sample:
  Muscle (Mouse) Lysate at 40 ug
  Primary: Anti- Podoplanin at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 15 kD
  Observed band size: 37 kD
  

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Podoplanin) Polyclonal Antibody, Unconjugated (at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！