种属反应性Human,Mouse,Rat,Dog,Cow,Horse,Rabbit,Sheep
验证应用WB,IHC-P,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human PDGF-B 155-210/241
偶联Non-conjugated
Anti-PDGF-B antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Secreted. Note=Released by platelets upon wounding.
其它名称PDGF beta
PDGF-B
Platelet-derived growth factor B-chain
PDGF AB + BB
Becaplermin
c sis
FLJ1285
Oncogene SIS
PDGF 1
PDGF 2
PDGF A
PDGF A chain
PDGF B chain
PDGF subunit A
PDGF subunit B
PDGF1
PDGF2
PDGFA
PDGFB
PDGFR1
Platelet derived growth factor 2
Platelet derived growth factor alpha
Platelet derived growth factor alpha chain
Platelet derived growth factor alpha polypeptide
Platelet derived growth factor B chain
Platelet derived growth factor beta
Platelet derived growth factor beta polypeptide
Platelet derived growth factor subunit A
Platelet derived growth factor subunit B
SIS
SSV
v sis platelet derived growth factor beta polypeptide.
more
Anti-PDGF-B antibody应用
WB:1:500-2000
IHC-P:1:400-800
FC:1μg/Test
Fig1: Blank control (blue line):Hela(blue).
Primary Antibody (green line): Rabbit Anti-PDGF-B antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Fig2: Sample:
Stomach (Rat) Lysate at 40 ug
Primary: Anti-PDGF-B at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 32 kD
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