Anti-PDE4D antibody

Anti-PDE4D antibody

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human PDE4D 401-550/809

• 偶联Non-conjugated

• Anti-PDE4D antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cytoplasm. Membrane. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Note=Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.

• 其它名称cAMP specific 3',5' cyclic phosphodiesterase 4D
  DKFZp686M11213
  FLJ97311
  cAMP specific 3' 5' cyclic phosphodiesterase 4D
  cAMP specific phosphodiesterase 4D
  cAMP specific phosphodiesterase PDE4D
  cAMP specific phosphodiesterase PDE4D6
  cyclic AMP phosphodiesterase
  DPDE 3
  DPDE3
  Dunce like phosphodiesterase E3
  HSPDE4D
  PDE 43
  PDE43
  PDE4DN2
  Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3)
  Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila)
  Phosphodiesterase 4D cAMP specific
  Phosphodiesterase 4D, cAMP specific (dunce)
  Phosphodiesterase E3 dunce homolog Drosophila
  STRK 1
  STRK1.

  more

• Anti-PDE4D antibody应用

  WB:1:500-2000
  FC:1μg/Test

• 

  Fig1: Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
  Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μgL 1X PBS containing 0.5% BSA(green).

  

  Fig2: Sample:Brain(Mouse) Lysate at 30 ug
  Primary: Anti-PDE4D at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
  Predicted band size: 90 kD
  Observed band size: 100 kD
  

  

  Fig3: Sample:Brain(Mouse) Lysate at 30 ug
  Primary: Anti-PDE4D  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
  Predicted band size: 90 kD
  Observed band size: 90kD
  

  

  Fig4: Sample:
  Cerebrum (Mouse) Lysate at 40 ug
  Primary: Anti- PDE4D at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 90kD
  Observed band size: 90 kD

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