种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit
验证应用WB,IHC-P,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human cytokeratins 1,2,4,5,6,7,8,71,72,75,78
偶联Non-conjugated
Anti-Pan Cytokeratin antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式Affinity purified by Protein A
亚细胞定位Cytoplasmic.
其它名称pan-cytokeratin
pan-CK
pan CK
P-CK
wide spectrum Cytokeratin
Cytokeratins
[cytokeratins 1,2,4,5,6,7,8,71,72,75,78].
more
Anti-Pan Cytokeratin antibody应用
WB:1:500-2000
IHC-P:1:400-800
FC:1μg /test
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Sample:Bladder (Mouse) Lysate at 40 ug
Primary: Anti-Pan Cytokeratin at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42-64 kD
Observed band size: 60 kD
Fig3: Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig4: Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti-Pan Cytokeratin antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Fig5: Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig6: Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated ( at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig7: Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30m
Fig8: Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at
Anti-Pan Cytokeratin antibody信息由上海泽叶生物科技有限公司为您提供,如您想了解更多关于Anti-Pan Cytokeratin antibody报价、型号、参数等信息,欢迎来电或留言咨询。除供应Anti-Pan Cytokeratin antibody外,上海泽叶生物科技有限公司还可为您提供Anti-Cytokeratin 17 antibody、Anti-14-3-3 beta/alpha antibody、Anti-Caveolin-1 antibody等产品,公司有专业的客户服务团队,是您值得信赖的合作伙伴。