Anti-Pancreatic Amylase antibody
种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit
验证应用WB,IHC-P
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human Pancreatic Amylase 151-250/511
偶联Non-conjugated
Anti-Pancreatic Amylase antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Secreted, extracellular space.
其它名称Alpha amylase
Alpha amylase pancreatic precursor
AMY2
AMY2A
AMY2B
Amylase alpha 2A
Amylase alpha 2A pancreatic
Amylase alpha 2B
Amylase alpha 2B pancreatic
Amylase pancreatic alpha 2A
Amylase pancreatic alpha 2B
Pancreatic alpha amylase
AMYP_HUMAN.
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Anti-Pancreatic Amylase antibody应用
WB:1:500-2000
IHC-P:1:400-800
Fig1: Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-Pancreatic Amylase at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Pancreatic Amylase) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Fig3: Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-Pancreatic Amylase at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 61 kD
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