Anti-P2Y8 antibody

Anti-P2Y8 antibody

• 种属反应性Human

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human P2Y8 131-230/359

• 偶联Non-conjugated

• Anti-P2Y8 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cell membrane; Multi-pass membrane protein.

• 其它名称P2RY8
  P2RY8_HUMAN
  P2Y purinoceptor 8
  P2Y8
  Purinergic receptor P2Y G protein coupled 8.

• Anti-P2Y8 antibody应用

  WB:1:500-2000
  FC:2ug/Test

• 

  Fig1: Sample:
  Raji Cell (Human) Lysate at 30 ug
  Primary: Anti- P2Y8  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 66 kD
  Observed band size: 50 kD
  

  

  Fig2: Blank control: HL60.
  Primary Antibody (green line): Rabbit Anti-P2Y8 antibody 
  Dilution: 2μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody (blue line): Goat anti-rabbit IgG-AF488
  Dilution: 1μg /test.
  Protocol
  The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

  

  Fig3: Blank control: Raji.
  Primary Antibody (green line): Rabbit Anti-P2Y8 antibody 
  Dilution: 2μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody : Goat anti-rabbit IgG-AF488
  Dilution: 1μg /test.
  Protocol
  The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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