Anti-Optineurin antibody

Anti-Optineurin antibody

• 种属反应性Human,Mouse,Rat,Cow,Sheep

• 验证应用WB

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human Optineurin 341-440/577

• 偶联Non-conjugated

• Anti-Optineurin antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式Affinity purified by Protein A

• 亚细胞定位Cytoplasm > perinuclear region. Golgi apparatus.

  
  

• 其它名称14.7K interacting protein
  Ag9 C5
  ALS12
  E3 14.7K interacting protein
  E3-14.7K-interacting protein
  FIP 2
  FIP-2
  FIP2
  Glaucoma 1 open angle E (adult onset)
  Glaucoma 1 open angle E
  GLC1E
  HIP 7
  HIP-7
  HIP7
  Huntingtin interacting protein 7
  Huntingtin interacting protein HYPL
  Huntingtin interacting protein L
  Huntingtin yeast partner L
  Huntingtin-interacting protein 7
  Huntingtin-interacting protein L
  HYPL
  Injury inducible protein I 55
  NEMO related protein
  NEMO-related protein
  NRP
  Optic neuropathy inducing protein
  Optic neuropathy-inducing protein
  Optineurin
  OPTN
  OPTN_HUMAN
  TFIIIA IntP
  TFIIIA-IntP
  Transcription factor IIIA interacting protein
  Transcription factor IIIA-interacting protein
  Tumor necrosis factor alpha inducible cellular protein containing leucine zipper domains.

  more

• Anti-Optineurin antibody应用

  WB:1:500-2000

• 

  Fig1: Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Optineurin) Polyclonal Antibody, Unconjugated (bs-13658R) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

  

  Fig2: Sample:
  Cerebrum (Mouse) Lysate at 40 ug
  Cerebellum (Mouse) Lysate at 40 ug
  Primary: Anti-Optineurin at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 66kD
  Observed band size: 66kD

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