Anti-Nucleoporin p62 antibody
种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit
验证应用WB,IHC-P,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human Nucleoporin p62 451-522/522
偶联Non-conjugated
Anti-Nucleoporin p62 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式Affinity purified by Protein A
亚细胞定位Nucleus, nuclear pore complex. Cytoplasm, cytoskeleton, spindle pole.
其它名称NUP62_HUMAN
62 kDa nucleoporin
DKFZp547L134
FLJ20822
FLJ43869
MGC841
Nuclear pore glycoprotein p62
nucleoporin 62kDa
nucleoporin p62
nucleoporin p62KD
NUP62
NUP62 protein
Nucleoporin Nup62
p62.
more
Anti-Nucleoporin p62 antibody应用
WB:1:500-2000
IHC-P:1:400-800
FC:1ug/Test
Fig1: Blank control (Black line):Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-Nucleoporin p62 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (Nucleoporin p62) Polyclonal/MonoclonalAntibody, Unconjugated at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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