Anti-NR1H4 antibody

Anti-NR1H4 antibody

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Sheep

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human FXR/Bile Acid Receptor NR1H4 175-280/486

• 偶联Non-conjugated

• Anti-NR1H4 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Nucleus.

• 其它名称Bile Acid Receptor NR1H4
  BAR
  FXR
  Farnesoid X activated receptor
  Farnesoid X receptor
  Farnesoid X-activated receptor
  Farnesol receptor HRR 1
  Farnesol receptor HRR-1
  Farnesol receptor HRR1
  FXR
  HRR 1
  HRR1
  NR1H4
  NR1H4_HUMAN
  Nuclear receptor subfamily 1 group H member 4
  Retinoid X receptor interacting protein 14
  Retinoid X receptor-interacting protein 14
  RIP 14
  RIP14
  RXR interacting protein 14
  RXR-interacting protein 14.

  more

• Anti-NR1H4 antibody应用

  WB:1:500-2000
  FC:3ug/test

• 

  Fig1: Sample:
  HepG2 (Human) Cell Lysate at 30 ug
  Primary: Anti-NR1H4  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 56 kD
  Observed band size: 56 kD
  

  

  Fig2: Sample:
  Large intestine (Mouse) Lysate at 40 ug
  Primary: Anti-NR1H4  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 56 kD
  Observed band size: 56 kD
  

  

  Fig3: Sample:
  Liver (Mouse) Lysate at 40 ug
  Primary: Anti-NR1H4) at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 56 kD
  Observed band size: 56 kD
  

  

  Fig4: Sample:
  Liver (Mouse) Lysate at 40 ug
  Primary: Anti- NR1H4  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 56 kD
  Observed band size: 56 kD
  

  

  Fig5: Blank control:A549.
  Primary Antibody (green line): Rabbit Anti-NR1H4 antibody
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody : Goat anti-rabbit IgG-PE
  Dilution: 3μg /test.
  Protocol
  The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

  

  Fig6: Sample: HepG2 Cell (Human) Lysate at 40 ug
  Primary: Anti-NR1H4  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 56 kD
  Observed band size: 56 kD

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