种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep
验证应用WB,IHC-P
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human NARG1 121-220/866
偶联Non-conjugated
Anti-NARG1 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Cytoplasm. Nucleus. Mainly cytoplasmic, nuclear in some cases. Present in the free cytosolic and cytoskeleton-bound polysomes, but not in the membrane-bound polysomes.
其它名称5730450D16Rik
6330400I15
ASTBDN
GA19
Gastric cancer antigen Ga19
MGC29428
mNAT1
N alpha acetyltransferase 15, NatA auxiliary subunit
N terminal acetyltransferase
N(alpha) acetyltransferase 15, NatA auxiliary subunit
N-alpha-acetyltransferase 15
N-terminal acetyltransferase
Naa15
NAA15_HUMAN
NatA auxiliary subunit
NATH
NMDA receptor regulated 1
NMDA receptor regulated protein 1
NMDA receptor-regulated protein 1
Protein tubedown 1
Protein tubedown-1
Tbdn 1
TBDN100
Transcriptional coactivator tubedown 100
tubedown-1.
more
Anti-NARG1 antibody应用
WB:1:500-2000
IHC-P:1:400-800
Fig1: Sample:
SP2/0 (Mouse) Lysate at 30 ug
Primary: Anti-NARG1 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 101 kD
Observed band size: 101 kD
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NARG1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NARG1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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