Anti-NADPH oxidase 4 antibody

Anti-NADPH oxidase 4 antibody

• 种属反应性Human,Mouse,Rat,Dog,Cow,Horse

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human Nox-4 81-180/578

• 偶联Non-conjugated

• Anti-NADPH oxidase 4 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction, focal adhesion. Cell membrane. Note=May localize to plasma membrane and focal adhesions.

  
  

• 其它名称KOX 1
  KOX
  Nox 4
  Nox-4
  NADPH oxidase 4
  RENOX
  Kidney oxidase-1
  Kidney superoxide-producing NADPH oxidase
  Kox-1
  NADPH
  Nox4
  NOX4_HUMAN
  Renal NAD(P)H-oxidase
  RENOX.

  more

• Anti-NADPH oxidase 4 antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg/Test

• 

  Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

  

  Fig3: Sample: Hela Cell Lysate at 40 ug
  Primary: Anti- NADPH oxidase 4 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 64 kD
  Observed band size: 64 kD
  

  

  Fig4: Sample: HepG2 Cell Lysate at 40 ug
  Primary: Anti- NADPH oxidase 4)

  

  Fig5: Sample: Lung (Mouse) Lysate at 40 ug
  Primary: Anti- NADPH oxidase4  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 64 kD
  Observed band size: 64 kD
  

  

  Fig6: Sample:
  Cerebellum (Mouse) Lysate at 40 ug
  Primary: Anti-NADPH oxidase 4  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 64 kD
  Observed band size: 64 kD
  

  

  Fig7: Blank control: Raji.
  Primary Antibody (green line): Rabbit Anti-NADPH oxidase 4 antibody 
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody : Goat anti-rabbit IgG-PE
  Dilutio

  

  Fig8: Sample:
  muscle (Mouse) Lysate at 40 ug
  Primary: Anti-NADPH oxidase 4  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 64 kD
  Observed band size: 64 kD
  

  

  Fig9: Sample:
  heart (Mouse) Lysate at 40 ug
  Primary: Anti-NADPH oxidase 4  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 64 kD
  Observed band size: 64 kD

•  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 64 kD
  Observed band size: 64 kD
  

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