Anti-mu Opioid receptor antibody
种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Guinea Pig
验证应用WB,IHC-P
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human mu Opioid receptor 165-270/400
偶联Non-conjugated
Anti-mu Opioid receptor antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式Affinity purified by Protein A
亚细胞定位Cell membrane; Multi-pass membrane protein. Isoform 12: Cytoplasm.
其它名称LMOR
MOR 1
mor
MOR1
Mu opiate receptor
mu type opioid receptor
Mu type opioid receptor MOR 1
muOR
Opioid receptor mu 1
Opioid receptor mu
OPRM
OPRM1
OPRM_HUMAN.
more
Anti-mu Opioid receptor antibody应用
WB:1:500-2000
IHC-P:1:400-800
Fig1: Sample:
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti-mu Opioid receptor at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45/53 kD
Fig2: Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-mu Opioid receptor at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 47 kD
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (mu Opioid receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (mu Opioid receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Fig5: Sample:
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti- mu Opioid receptor at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 47 kD
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