Anti-MHC Class II antibody

Anti-MHC Class II antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human HLA class II histocompatibility antigen DM beta chain 111-200/263

• 偶联Non-conjugated

• Anti-MHC Class II antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cell membrane. Endoplasmic reticulum membrane.

• 其它名称DMA
  DMB
  DRB
  HLA class II histocompatibility antigen DM beta chain
  HLA DMB
  HLADM
  HLADP1B
  Major histocompatibility complex clas
  HLA class II histocompatibility antigen, DM beta chain
  MHC class II antigen DMB
  Really interesting new gene 7 protein
  HLA-DMB
  RING7
  DMB_HUMAN
  MHC2.

  more

• Anti-MHC Class II antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Protein: mouse lung lysates at 30ug;
  Primary: Anti-MHC Class II  at 1:200;
  Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000;
  ECL excitated the fluorescence;
  Predicted band size : 27kD
  Observed band size : 30kD
  

  

  Fig2: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-MHC Class II Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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