Fig1: Blank control(blue): 293T Cells(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody: Rabbit Anti-ITM2A/AF488 Conjugated antibody , Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.