种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Zebrafish,Sheep
验证应用WB,IHC-P,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human HNF1 201-350/628
偶联Non-conjugated
Anti-HNF1A antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Nucleus. [ALTERNATIVE PRODUCTS] Event=Alternative splicing; Named isoforms=3; Name=A; IsoId=P20823-1; Sequence=Displayed; Name=B; IsoId=P20823-2; Sequence=VSP_002250, VSP_002251; Name=C; IsoId=P20823-3; Sequence=VSP_002252, VSP_002253.
其它名称HNF1A
TCF1
HNF1 alpha
HNF-1 Alpha
Hepatocyte nuclear factor 1 Alpha
Albumin proximal factor
Hepatic nuclear factor 1 alpha
Hepatic nuclear factor 1
Hepatic transcription factor 1 alpha
Hepatic transcription factor 1
HNF 1
HNF 1A
HNF1A
Interferon production regulator factor
LF B1
LF B1 hepatic nuclear factor
LFB 1
LFB1
LFB1 hepatic nuclear factor
Liver specific transcription factor LF B1
Liver specific transcription factor LFB1
Maturity onset diabetes of the young 3
MODY 3
MODY3
TCF 1
TCF1
TCF-1
Transcription factor 1
Transcription factor 1 hepatic
HNF1A_HUMAN.
more
Anti-HNF1A antibody应用
WB:1:500-2000
IHC-P:1:400-800
FC:3ug/test
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HNF1A) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HNF1A) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig3: Sample:
HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti-HNF1 (Bs-1405R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 67 kD
Observed band size: 67 kD
Fig4: Blank control:A549.
Primary Antibody (green line): Rabbit Anti-HNF1A antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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