种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep,Macaque Monkey
验证应用WB,IHC-P
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human HIPK1 1-100/1210
偶联Non-conjugated
Anti-HIPK1 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Nucleus. Cytoplasm. Predominantly nuclear.
其它名称Hipk 1
Hipk1
HIPK1_HUMAN
Homeodomain interacting protein kinase 1
Homeodomain interacting protein kinase 1 like protein
Homeodomain-interacting protein kinase 1
Myak
Nbak 2
Nbak2
Nuclear body associated kinase 2
Nuclear body associated kinase 2b.
more
Anti-HIPK1 antibody应用
WB:1:500-2000
IHC-P:1:400-800
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HIPK1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Sample:
Mcf-7(Human) Cell Lysate at 40 ug
Primary: Anti-HIPK1at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kD
Observed band size: 131kD
Anti-HIPK1 antibody信息由上海泽叶生物科技有限公司为您提供,如您想了解更多关于Anti-HIPK1 antibody报价、型号、参数等信息,欢迎来电或留言咨询。除供应Anti-HIPK1 antibody外,上海泽叶生物科技有限公司还可为您提供Anti-α-Actin (cardiac actin) antibody、Anti-Cytokeratin 17 antibody、Anti-ESR1 antibody等产品,公司有专业的客户服务团队,是您值得信赖的合作伙伴。