Anti-GPR78 antibody

Anti-GPR78 antibody

• 种属反应性Human

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human GPR78 201-300/363

• 偶联Non-conjugated

• Anti-GPR78 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cell membrane; Multi-pass membrane protein.

• 其它名称GPCR GPR78
  G protein-coupled receptor 78
  G protein coupled receptor GPR78
  G-protein coupled receptor 78
  GPR78
  GPR78_HUMAN
  PRO19818
  UNQ5925.

  more

• Anti-GPR78 antibody应用

  WB:1:500-2000
  FC:1μg /test

• 

  Fig1: Sample:
  A549 Cell (Human) Lysate at 30 ug
  Primary: Anti-GPR78at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 39 kD
  Observed band size: 39 kD
  

  

  Fig2: Blank control: 293T Cells(blue).
  Primary Antibody: Rabbit Anti-GPR78/AF488 Conjugated antibody , Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
  Protocol
  The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody for 30 min on ice. Acquisition of 20,000 events was performed.

  

  Fig3: Blank control (blue line): U937 (blue).
  Primary Antibody (green line): Rabbit Anti-GPR7 antibody 
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
  Dilution: 1μg /test.
  Protocol
  The cells were fixed with 2% paraformaldehyde for 10 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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