Anti-Glucagon Receptor antibody
种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Guinea Pig
验证应用WB,IHC-P,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human Glucagon Receptor 11-110/477
偶联Non-conjugated
Anti-Glucagon Receptor antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Cell membrane; Multi-pass membrane protein.
其它名称GCGR
GGR
GL R
GL-R
GLR
GLR_HUMAN
MGC138246
Glucagon receptor precursor.
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Anti-Glucagon Receptor antibody应用
WB:1:500-2000
IHC-P:1:400-800
FC:1μg/Test
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Paraformaldehyde-fixed, paraffin embedded (human stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig3: Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody: Rabbit Anti-Glucagon Receptor/AF647 Conjugated antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Fig4: Sample: Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-Glucagon Receptor at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Fig5: Sample:Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Glucagon Receptorat 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 51kD
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