Anti-GLK antibody
种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Horse,Rabbit
验证应用WB,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human GCK 101-200/465
偶联Non-conjugated
Anti-GLK antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
其它名称Glucokinase
ATP D hexose 6 phosphotransferase
GCK
GK
Glucokinase isoform 1
Hexokinase 4
Hexokinase D
Hexokinase D pancreatic isozyme
Hexokinase type IV
Hexokinase4
HexokinaseD
HHF 3
HHF3
HK 4
HK IV
HK4
HXKP
Maturity onset diabetes of the young 2
MODY 2
MODY2
GLUKA
RNGK2
HXK4_HUMAN.
more
Anti-GLK antibody应用
WB:1:500-2000
FC:3ug/Test
Fig1: Protein: rat liver lysates, 30ug;
Primary: Anti-GLat 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000;
ECL excitated the fluorescence;
Predicted band size : 51kD
Observed band size : 51kD
Fig2: Blank control: A431.
Primary Antibody (green line): Rabbit Anti-GLK antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Fig3: Sample:
Panc-1(Human) Cell Lysate at 30 ug
Primary: Anti-GLK at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
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