Anti-Gamma-Adaptin antibody

Anti-Gamma-Adaptin antibody

• 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Rabbit

• 验证应用WB,IHC-P,ICC/IF

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human Gamma-Adaptin 721-822/822

• 偶联Non-conjugated

• Anti-Gamma-Adaptin antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side.

• 其它名称Adapter-related protein complex 1 subunit gamma-1
  Adaptor protein complex AP 1 gamma 1 subunit
  Adaptor protein complex AP 1 subunit gamma 1
  Adaptor protein complex AP-1 subunit gamma-1
  Adaptor related protein complex 1 gamma 1 subunit
  adaptor-related protein complex 1 gamma 1 subunit
  ADTG
  ADTG
  AP 1 complex subunit gamma 1
  AP-1 complex subunit gamma-1
  AP1G1
  AP1G1_HUMAN
  CLAPG1
  CLAPG1
  Clathrin assembly protein complex 1 gamma 1 large chain
  Clathrin assembly protein complex 1 gamma large chain
  Clathrin assembly protein complex 1 gamma-1 large chain
  Clathrin associated/assembly/adaptor protein large gamma 1
  clathrin-associated/assembly/adaptor protein large gamma 1
  Gamma1 adaptin
  Gamma1-adaptin
  Golgi adaptor HA1/AP1 adaptin gamma subunit
  Golgi adaptor HA1/AP1 adaptin subunit gamma 1
  Golgi adaptor HA1/AP1 adaptin subunit gamma-1
  MGC18255.

  more

• Anti-Gamma-Adaptin antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  IF:1:100-500
  ICC/IF:1:100-500

• 

  Fig1: Tissue/cell: Rat brian; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti- Gamma-Adaptin, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig2: Tissue/cell: rat lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig3: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig4: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

  

  Fig5: Sample:
  293T(Human) Cell Lysate at 30 ug
  Jurkat(Human) Cell Lysate at 30 ug
  Primary: Anti-Gamma-Adaptin  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 91 kD
  Observed band size: 91 kD

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